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| Registros recuperados: 124 | |
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| Pfeiffer,C.P.; Britto,L.R.G.. |
| The calcium-binding proteins calbindin (CB), calretinin (CR), and parvalbumin (PV) have been extensively studied over the last decade since they appear to be important as buffers of intracellular calcium. In the present study we investigated the distribution of these proteins in the chick visual system by means of conventional immunocytochemistry. The results indicated that CB, CR, and PV are widely distributed in retinorecipient areas of the chick brain. In some regions, all three calcium-binding proteins were present at different intensities and often in different neurons such as in the dorsolateral thalamic complex. In other areas, such as the nucleus geniculatus lateralis ventralis, only CB and CR were detected, whereas PV was absent. These results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calbindin; Calretinin; Chick; Immunohistochemistry; Parvalbumin; Visual system. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100009 |
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| Cassali,G.D.; Salvador,A.; Freitas,C.; Dutra,A.P.; Schmitt,F.C.. |
| Flow cytometric analysis of DNA content was performed on 28 samples of canine mammary tumors. Nine of them were benign and 19 were malignant. All benign tumors and 11 malignant tumors (57.9%) were diploid (P<0.05). Form the aneuploid tumors, five (26.3%) were hyperdiploid, one (5.3%) hypodiploid, one (5.3%) near triploid and one (5.3%) multiploid. The analysis of the expression of the markers PR and CD31 revealed a significant difference between diploid and aneuploid tumors (P<0.05). The immunoreactivity of PR was higher in diploid tumors, while the immunoreactivity of CD31 was stronger in aneuploid tumors. No difference between the markers MIB-1, c-erbB2, p53 and Cyclin D1 was observed (P>0.05). Using the flow cytometry analysis and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Breast cancer; Neoplasm; DNA flow cytometry; Immunohistochemistry. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500011 |
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| Take,G.; Erdogan,D.; Helvacioglu,F.; Göktas,G.; Ozbey,G.; Uluoglu,C.; Yücel,B.; Guney,Y.; Hicsonmez,A.; Ozkan,S.. |
| The effect of ionizing irradiation on testes and the protective effects of melatonin were investigated by immunohistochemical and electron microscopic methods. Eighty-two adult male Wistar rats were divided into 10 groups. The rats in the irradiated groups were exposed to a sublethal irradiation dose of 8 Gy, either to the total body or abdominopelvic region using a 60Co source at a focus of 80 cm away from the skin in the morning or evening together with vehicle (20% ethanol) or melatonin administered 24 h before (10 mg/kg), immediately before (20 mg/kg) and 24 h after irradiation (10 mg/kg), all ip. Caspace-3 immunoreactivity was increased in the irradiated group compared to control (P < 0.05). Melatonin-treated groups showed less apoptosis as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Testes; Gamma-irradiation; Melatonin; Immunohistochemistry; Electron microscopy. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700006 |
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| Furuoka, Hidefumi; Yabuzoe, Atushi; Horiuchi, Motohiro; Tagawa, Yuichi; Yokoyama, Takashi; Yamakawa, Yoshio; Shinagawa, Morikazu; Sata, Tetsutaro. |
| For immunohistochemistry of the prion diseases, several pretreatment methods to enhance the immunoreactivity of human and animal abnormal proteinase-resistant prion protein (PrPSc) on the tissue sections have been employed. The method of 121 degrees C hydrated autoclaving pretreatment or the combination method of 121 degrees C hydrated autoclaving with a certain chemical reagent ( formic acid or proteinase K, etc) are now widely used. We found that an improved hydrated autoclaving method at 135 degrees C, more effectively enhanced PrPSc immunoreactivity for the antibodies recognizing the linear epitope. In addition, this method was more effective for the long-term fixation samples as compared with other previous methods. However, this modified method could... |
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Palavras-chave: Prion protein; Immunohistochemistry; Antigen retrieval; Autoclaving; Monoclonal antibody; CREUTZFELDT-JAKOB-DISEASE; MONOCLONAL-ANTIBODIES; IMMUNOCYTOCHEMISTRY; IMMUNOREACTIVITY; PROTOCOLS; FIXATION; DEPOSITS; PLAQUES; BRAIN; PRP. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/712 |
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| Araújo,Caliandra Pinto; Xavier,Flávia Caló de Aquino; Gurgel,Clarissa Araújo Silva; Ramos,Eduardo Antônio Gonçalves; Freitas,Valéria Souza; Schlaepfer-Sales,Caroline Brandi; Ramalho,Luciana Maria Pedreira; dos Santos,Jean Nunes. |
| The extracellular matrix (ECM) plays an important role in the regulation of biological events such as the development of cell migration, proliferation and differentiation. Chronic sun exposure causes changes present in the ECM of actinic cheilitis (AC), a premalignant lesion of the lower lip which helps to understand the carcinogenesis of the lip. This study aimed to investigate elastin, the main component of solar elastosis alternating current in an attempt to establish the relationship between this protein and ECM in epithelial dysplasia. Paraffin embedded tissue sections of the lesions of 35 cases of AC were analyzed by immunohistochemistry for elastin, and became the association with the degree of epithelial dysplasia and age. Highest scores of elastin... |
| Tipo: Journal article |
Palavras-chave: Matrix extracellular; Elastin; Immunohistochemistry; Actinic cheilitis. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200044 |
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| Amaral,A.V.C.; Silva,G.A.; Costa,A.P.A.; Oliveira,K.S.; Jordão,P.; Ribeiro,A.P.; Renzo,R.; Laus,J.L.. |
| Sildenafil citrate is a type-5 phosphodiesterase inhibitor (PDE-5), able to inhibit type-6 phosphodiesterase (PDE-6) as well, providing clinical benefits and paraeffects, some of them potentially related to the retina. The effects of the sildenafil on the retrobulbar and retinal circulation were studied in 27 adult male rabbits of the White New Zealand breed. The electric activity of the retina was evaluated before and at the end of the treatments, and immunohistochemistry studies were conducted. An amplitude increase of the b wave was found in the mixed response of cones and rods after 7 days of treatment with sildenafil citrate. However, in the other evaluations and periods, the values did not differ from the basal ones. Through immunohistochemistry, no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabbit; Sildenafil citrate; Electroretinography; Immunohistochemistry. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601589 |
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| Yildirim,Aysegul Burcin; Karabulut,Derya; Dundar,Munis; Ulusoy,Hasan Basri; Sonmez,Mehmet Fatih. |
| The aim of this work was to study the relative ghrelin and growth hormone secretagogue receptor (GHS-R)1a gene expression in the kidney of long-term diabetic rats. Forty male Wistar albino rats were divided into four groups: C- control group, DI- one month diabetic rats group, DII- two months diabetic rats group, and DIII- three months diabetic rats group. Diabetes was induced by streptozotocin STZ (40mg/kg i.p). The rats were decapitated under ketamine anesthesia and their kidney tissues were removed. Tissue GHS-R mRNA levels, ghrelin expression, and histopathological damage scores were compared. Dilatation in the distal tubules, epithelial desquamation into the lumen of the tubules and transparent tubules showing glycogen vacuolation were observed in all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diabetes mellitus; Ghrelin; GHSR-1a; Kidney; Immunohistochemistry; RT-PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100300 |
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| Laskoski,Luciane Maria; Valadão,Carlos Augusto Araújo; Vasconcelos,Rosemeri de Oliveira; Ferrucci,Danilo; Silva,Juliete Aparecida Francisco; Machado,Dagmar Ruth Stach; Doria,Renata Gebara Sampaio; Mendonça,Fabio de Sousa. |
| Twenty horses were used in the experiment, for composed control group, (Cg) instrumented group, (Ig;without intestinal obstruction), treated group (Tg;submitted to intestinal obstruction and hydrocortisone treatment) and non-treated group (Ntg;submitted to intestinal obstruction without treatment). Immunohistochemistry and zymography techniques were used for researches on MMPs 2 and 9 in horse hoof laminae. There was an increase in the expression of MMP-2 in animals of Tg and Ntg. MMP-9 increased on animals from groups Ntg and Ig, however there was no rise of this MMP on the Tg when compared to the other groups in the immunohistochemistry analysis. Based on the results, it was observed that the intestinal injury caused by enterotomy and intestinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laminitis; Metalloprotease; Horse; Zymography; Immunohistochemistry. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100012 |
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| Ongaratti,B.R.; Silva,C.B.O.; Trott,G.; Haag,T.; Leães,C.G.S.; Ferreira,N.P.; Oliveira,M.C.; Pereira-Lima,J.F.S.. |
| Meningiomas are common, usually benign tumors of the central nervous system that have a high rate of post-surgical recurrence or regrowth. We determined expression of the proteins merlin, NDRG2, ERBB2, and c-MYC in meningiomas using immunohistochemistry and assessed relationships between protein expression and gender, age, tumor grade, and recurrence or regrowth. The study sample comprised 60 patients, (44 women and 16 men) with a mean age of 53.2±12.7 years. Tumors were classified as grade I (n=48) or grades II and III (n=12). Expression of merlin, NDRG2, ERBB2, and c-MYC was not significantly different statistically with relation to gender, age, or meningioma recurrence or regrowth. Merlin was expressed in 100% of the cases. No statistically significant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Meningioma; Merlin; NDRG2; ERBB2; C-MYC; Immunohistochemistry. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400706 |
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| Gutiérrez Cantú,Francisco Javier; Mariel Cárdenas,Jairo; Oliva Rodríguez,Ricardo; Romo Ramírez,Gabriel Fernando; Sánchez Méraz,Wulfrano; Mariel Murga,Humberto; Leal Tobias,Luis Armando. |
| During experiments in animal studies, it has been observed that enamelysin (MMP-20) is expressed during tooth development in the late secretory stage of amelogenesis but not in the mature enamel.The aim of this research was to determine the location of MMP-20 in human tooth germs in the different structures of the enamel organ.The detection of MMP-20 was performed by immunohistochemistry in 20 specimens obtained from human fetuses. Immunostaining of MMP-20 was observed from the presecretor stadium in stellate reticulum and intermediate stratum and in the basal portion of ameloblasts in the secretory stage in stellate reticulum, stratum intermedium, secretory ameloblasts, odontoblasts and dental papilla. The results of this research show the location of... |
| Tipo: Journal article |
Palavras-chave: Enamelysin; Immunohistochemistry; Ameloblasts; Odontoblasts. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400022 |
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| Emam,Mahmoud Abdelghaffar; El-Shafey,Anwar A; Kassab,Ahmed A. |
| Fourteen vomeronasal organs (VNOs) of adult males one-humped camels (Camelus dromedarius) and Egyptian water buffaloes (Bubalus bubalis) (n=7/each) were examined immunohistochemically with neuronal markers; synaptophysin (SYP) and glial fibrillary acidic protein (GFAP) to clarify the distribution of the vomeronasal (VN) receptor cells and nerve fibers, in addition to elucidate the existence of non-neuronal elements via S-100 and endothelial nitric oxide synthase (eNOS). In both animals, the VNO was lined medially with VN sensory (olfactory) epithelium and non-sensory (respiratory) epithelium laterally. Immunohistochemically, both animals showed SYP immunolabeling only in the receptor cells of VN sensory epithelium while GFAP labeled the ensheathing cells... |
| Tipo: Journal article |
Palavras-chave: Vomeronasal organ; Camel; Buffalo; Immunohistochemistry. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400006 |
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| Guo,Jie; Wang,Lue; Xu,Haihua; Che,Xiaoxia. |
| BACKGROUND: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. RESULTS: Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits α1, α2, α3, α5,... |
| Tipo: Journal article |
Palavras-chave: Non-neuronal cholinergic system; Maxilla; Real-Time PCR; Immunohistochemistry. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100066 |
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| Oliveira,M.C.; Marroni,C.P.; Pizarro,C.B.; Pereira-Lima,J.F.; Barbosa-Coutinho,L.M.; Ferreira,N.P.. |
| Inactivating mutations of TP53, a tumor suppressor gene, are associated with abnormal cell proliferation. Although p53 expression is common in many human malignancies, p53 protein has seldom been evaluated in pituitary tumors. When detected, the percentage of p53-positive cells is low, and, in general, it is exclusive for invasive lesions. The aim of the present study was to use immunohistochemistry to determine the presence of p53 protein in pituitary adenomas from tumor samples of 163 surgeries performed in 148 patients (40% male, 60% female). In 35% of the cases the adenoma was nonfunctional, while in the others it was associated with PRL, GH and/or ACTH endocrine hypersecretion syndrome. Macroadenomas were observed in 83.2% of the cases with available... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Pituitary; Pituitary adenoma; Invasive adenoma; P53; Immunohistochemistry. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500008 |
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| Pereira,Paula Reis; Tagliari,Nelson Junior; Leite-Filho,Ronaldo Viana; Schaefer,Gabriela da Cruz; Costa,Fernanda Vieira Amorim da; Pavarini,Saulo Petinatti. |
| ABSTRACT: Neuroblastic tumors can originate from the central neuraxis, olfactory epithelium, adrenal medullary region or autonomous system. Ganglioneuroblastoma are a type of neuroblastic tumor, with very few case descriptions in animals. Diagnosis of facial nerve ganglioneuroblastoma was made in a feline leukemia virus-positive 11-month-old cat. The cat had hyporexia, left head tilt, depressed mental state, horizontal nystagmus, inability to retract the pinched left lip, anisocoria, ptosis, and absence of the menace reflex. Gross necropsy showed a mass at the left facial nerve root region. Histological examination of this mass showed neoplastic proliferation of neuroblasts arranged in a cohesive pattern and mature ganglion cells. Ganglion cells were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neuronal neoplasm; Cranial nerve VII; Immunohistochemistry; Feline leukemia virus.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000500551 |
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| GIACÓIA,Márcia Regina; MAIORKA,Paulo César; OLIVEIRA,Clair Motos; SINHORINI,Idércio Luiz; DAGLI,Maria Lúcia Zaidan. |
| The clinical, gross and microscopical features of a granulosa cell tumour in the ovary of a 2-year-old cat are described. This is a rare tumour in cats, moreover because of the animals age. It was diagnosed clinically by ultrasound examination. The tumour, a cystic mass in the region of the left ovary, metastasized to the epiplon and lungs. Tumour cells expressed vimentin and were arranged in a diffuse sarcomatous pattern, supported by a fine fibrovascular stroma. The presence of hair loss and repeated oestrus as clinical signs are indicative of orthoendocrine paraneoplastic syndrome, due to excessive oestrogen stimulation. This tumour should be considered in the differential diagnosis of behavioural disturbances in female cats. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Granulosa cell tumour; Ovary; Cats; Immunohistochemistry; Paraneoplastic syndromes. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000500005 |
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| Registros recuperados: 124 | |
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